Journal: Immunity

Article Title: Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans

doi: 10.1016/j.immuni.2020.07.003

Figure Lengend Snippet:

Article Snippet: Mouse anti-human CD161 PE-Cy7, clone HP-3G10 , Thermo Fisher Scientific , Cat# 25-1619-42; RRID: AB_10807086.

Techniques: Purification, Recombinant, Concentration Assay, Saline, Staining, Antibody Labeling, RNA Sequencing, Software, Sterility

Journal: PLoS ONE

Article Title: Enhanced Th1/Th17 Functions of CD161 + CD8 + T Cells in Mucosal Tissues of Rhesus Macaques

doi: 10.1371/journal.pone.0157407

Figure Lengend Snippet: (A) Representative flow plots showing gating strategy used for detection of CD161 + CD8 + T cells. (B) CD161 + T cell frequencies in PBMC of healthy rhesus macaques (n = 11). (C) Subset distribution of circulating CD161 + T cells based on the expression of CD4 and CD8 co-receptors in healthy rhesus macaques (n = 11). Asterisks denote significant differences calculated by Wilcoxon matched-pairs signed rank test (*** p<0.001).

Article Snippet: For immunophenotyping analysis PBMC were stained with the antibodies CD45 BV510 (BD clone D058-1283), CD3 APC-Cy7 (BD clone SP34 clone), CD4 QDot 605 and CD8 QDot 655 (NHP Reagent resource), CD161 PE-Cy7 (eBioscience clone HP-3G10), TCR γδ FITC (BD clone B1), TCR Vα7.2 BV421 (Biolegend clone 3C10), PBS-57 loaded CD1dTM APC (NHP Reagent resource), CD28 ECD (28.2 clone; Immunotech), and CD95 APC (BD clone DX2).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Enhanced Th1/Th17 Functions of CD161 + CD8 + T Cells in Mucosal Tissues of Rhesus Macaques

doi: 10.1371/journal.pone.0157407

Figure Lengend Snippet: (A) Frequencies of expression of the trafficking markers α4β7, CD69, CXCR3, CXCR5, CCR5, CCR6, and IL-18Rα by ex vivo CD161 + CD8 + T cells in the circulation of healthy rhesus macaques (Mean and SEM shown for data obtained from six animals). (B) Heat map showing relative expression of the chemokine receptors CCR5, CCR6, CXCR3, and CXCR5 generated using Prism 6 software. (C) Pie charts and arcs showing the proportion of different combinations of the chemokine receptors on CD161 + CD8 + T cells as analyzed by Pestle and SPICE 5.3. (D) Boolean analysis showing the range and mean frequencies of CCR5, CCR6, CXCR3, and CXCR5 by CD161 + CD8 + T cells in the 6 rhesus macaques.

Article Snippet: For immunophenotyping analysis PBMC were stained with the antibodies CD45 BV510 (BD clone D058-1283), CD3 APC-Cy7 (BD clone SP34 clone), CD4 QDot 605 and CD8 QDot 655 (NHP Reagent resource), CD161 PE-Cy7 (eBioscience clone HP-3G10), TCR γδ FITC (BD clone B1), TCR Vα7.2 BV421 (Biolegend clone 3C10), PBS-57 loaded CD1dTM APC (NHP Reagent resource), CD28 ECD (28.2 clone; Immunotech), and CD95 APC (BD clone DX2).

Techniques: Expressing, Ex Vivo, Generated, Software

Journal: PLoS ONE

Article Title: Enhanced Th1/Th17 Functions of CD161 + CD8 + T Cells in Mucosal Tissues of Rhesus Macaques

doi: 10.1371/journal.pone.0157407

Figure Lengend Snippet: (A) Frequency of cytokine-secreting CD161 + CD8 + T cells, CD161 – CD8 + T cells, and CD4 + T cells in mitogen-stimulated PBMC of uninfected rhesus macaques (n = 6–7). Intracellular cytokine staining was performed on freshly isolated cells stimulated for 16h with PMA/Ionomycin. Data shows mean and SEM after subtraction of background in unstimulated cells incubated with medium alone. (B) Pie charts and arcs showing the expression of different combinations of the cytokines IFN-γ, TNF-α, and IL-17 by CD161 + CD8 + T cells as analyzed by Pestle and SPICE 5.3. (C) Frequency of IFN-γ + IL-17 + co-producing cells in CD161 + CD8 + T cell and CD161 – CD8 + T cell subsets respectively. Asterisks denote significant difference (p = 0.03) calculated by the Wilcoxon matched-pairs signed rank test.

Article Snippet: For immunophenotyping analysis PBMC were stained with the antibodies CD45 BV510 (BD clone D058-1283), CD3 APC-Cy7 (BD clone SP34 clone), CD4 QDot 605 and CD8 QDot 655 (NHP Reagent resource), CD161 PE-Cy7 (eBioscience clone HP-3G10), TCR γδ FITC (BD clone B1), TCR Vα7.2 BV421 (Biolegend clone 3C10), PBS-57 loaded CD1dTM APC (NHP Reagent resource), CD28 ECD (28.2 clone; Immunotech), and CD95 APC (BD clone DX2).

Techniques: Staining, Isolation, Incubation, Expressing

Journal: PLoS ONE

Article Title: Enhanced Th1/Th17 Functions of CD161 + CD8 + T Cells in Mucosal Tissues of Rhesus Macaques

doi: 10.1371/journal.pone.0157407

Figure Lengend Snippet: (A) CD161 + CD8 + T cell frequencies in matched samples from PBMC, mesenteric lymph nodes (mes LN), colon and lung tissue of rhesus macaques (n = 6). (B) CD69 expression on CD161 + CD8 + T cells in PBMC, mes LN, colon and lung. (C) Frequencies of MAIT cells (CD161 + Vα7.2 + T cells), γδ T cells (pan TCR γδ + ), and iNKT (PBS-57-loaded CD1d TM + ) cells respectively in CD161 + CD8 + T cells of un-infected rhesus macaques. Asterisks denote significant differences (p = 0.03) calculated by the Wilcoxon matched-pairs signed rank test.

Article Snippet: For immunophenotyping analysis PBMC were stained with the antibodies CD45 BV510 (BD clone D058-1283), CD3 APC-Cy7 (BD clone SP34 clone), CD4 QDot 605 and CD8 QDot 655 (NHP Reagent resource), CD161 PE-Cy7 (eBioscience clone HP-3G10), TCR γδ FITC (BD clone B1), TCR Vα7.2 BV421 (Biolegend clone 3C10), PBS-57 loaded CD1dTM APC (NHP Reagent resource), CD28 ECD (28.2 clone; Immunotech), and CD95 APC (BD clone DX2).

Techniques: Expressing, Infection

Journal: PLoS ONE

Article Title: Enhanced Th1/Th17 Functions of CD161 + CD8 + T Cells in Mucosal Tissues of Rhesus Macaques

doi: 10.1371/journal.pone.0157407

Figure Lengend Snippet: (A) Intracellular levels of TNF-α and IL-17 cytokines by mitogen-stimulated CD161 + CD8 + T cells in PBMC, colon and lung of uninfected rhesus macaques (n = 5–6). Intracellular cytokine staining was performed on freshly isolated cells stimulated for 16h with PMA/Ionomycin. Data shows mean and SEM after subtraction of background in unstimulated cells incubated with medium alone. (B) Ratios of IL-17 to TNF-α in CD161 + CD8 + T cells isolated from PBMC, colon and lung showing increase in Th17-type responses in mucosal tissues. Asterisks denote significant differences (p<0.05) calculated by the Wilcoxon matched-pairs signed rank test.

Article Snippet: For immunophenotyping analysis PBMC were stained with the antibodies CD45 BV510 (BD clone D058-1283), CD3 APC-Cy7 (BD clone SP34 clone), CD4 QDot 605 and CD8 QDot 655 (NHP Reagent resource), CD161 PE-Cy7 (eBioscience clone HP-3G10), TCR γδ FITC (BD clone B1), TCR Vα7.2 BV421 (Biolegend clone 3C10), PBS-57 loaded CD1dTM APC (NHP Reagent resource), CD28 ECD (28.2 clone; Immunotech), and CD95 APC (BD clone DX2).

Techniques: Staining, Isolation, Incubation